研究成果（2008年－2017年）
- ◆学術論文 ◆産業財産権
T. Ushigusa, Y. Koyama, T. Ito, K. Watanabe, J. Chambers, A. Hasegawa, K. Uchida, R. Kanegi, S. Hatoya, T. Inaba, K. Sugiura, Innate immunity mediated by dendritic cells/macrophages plays a central role in the early period in tumor treatment using gene of Mycobacterium tuberculosis antigen, J Vet Med Sci. 80, 190-196, 2018
T. Ito, M. Eriguchi, Y. Koyama, Effect of pDNA complex particle size on gene expression: difference between in vitro and in vivo experiments, J Nanomater Mol Nanotechnol, 5:6, 2016.
DOI: 10.4172/2324-8777.1000202
Y. Koyama, T. Ito, A. Hasegawa, M. Eriguchi, T. Inaba, T. Ushigusa, K. Sugiura, Exosomes derived from tumor cells genetically modified to express Mycobacterium tuberculosis antigen: a novel vaccine for cancer therapy, Biotechnol Lett. 2016,. DOI: 10.1007/s10529-016-2185-1
Y. Koyama, C. Yoshihara, T. Ito, Novel Antitumor Strategy Utilizing a Plasmid Expressing a Mycobacterium tuberculosis Antigen as a “Danger Signal”to Block Immune Escape of Tumor Cells, Pharmaceutics, 7, 165-174, 2015
Y. Koyama, K. Sugiura, C. Yoshihara, T. Inaba, T. Ito, Highly Effective Non-Viral Antitumor Gene Therapy System Comprised of Biocompatible Small Plasmid Complex Particles Consisting of pDNA, Anionic Polysaccharide, and Fully Deprotected Linear Polyethylenimine, Pharmaceutics, 7, 152-164, 2015
T. Ito, M. Eriguchi, Y. Koyama, Bioabsorbable bioadhesive hydrogel comprising poly(acrylic acid) and poly(vinylpyrrolidone) for adhesion barrier and hemostatic device, MRS Communications, 5 , 291–295, 2015
T. Ito, Y. Koyama, M. Otsuka, Preparation of calcium phosphate nanocapsule including DNA/PEI/hyaluronic acid ternary complex for durable gene delivery, Journal of Pharmaceutical Sciences, 103, 179-184, 2014
C. Yoshihara, K. Hamada, M. Kuroda, Y. Koyama, Oncolytic plasmid: A novel strategy for tumor immuno-gene therapy, Oncol Lett, 3, 387–390, 2012
K. Hamada, C. Yoshihara, T. Ito, K. Tani, M. Tagawa, N. Sakuragawa, H. Itoh, Y. Koyama, Antitumor effect of chondroitin sulfate-coated ternary GMCSF plasmid complex for ovarian cancer, The Journal of Gene Medicine, 14, 120-127, 2012
K. Hagiwara, M. Nakata, Y. Koyama, T. Sato, The effects of coating pDNA/chitosan complexes with chondroitin sulfate on physicochemical characteristics and cell transfection, Biomaterials, 33, 7251–7260, 2012
T. Ito, Y. Koyama, M. Otsuka, DNA complex-releasing system by injectable self-setting apatite cement, The Journal of Gene Medicine, 14, 251-261, 2012
T. Ito, C. Yoshihara, K. Hamada, Y. Koyama, DNA/polyethyleneimine/hyaluronic acid small complex particles and tumor suppression in mice, Biomaterials, 31, 2912-2918, 2010,
C. Yoshihara, C. Yang Shew, T. Ito, Y. Koyama, Loosening of DNA/polycation Complexes by Synthetic Polyampholyte to Improve the Transcription Efficiency: Effect of Charge Balance in the polyampholyte, Biophysical Journal, 98, 1257-1266, 2010
C. Yoshihara, K. Hamada, Y. Koyama, Preparation of a novel adenovirus formulation with artificial envelope of multilayer polymer-coatings: Therapeutic effect on metastatic ovarian cancer, Oncology Reports, 23, 733-738, 2010
T. Ito, Y. Koyama, M. Otsuka, Analysis of the surface structure of DNA/polycation/hyaluronic acid ternary complex by Raman microscopy, Journal of Pharmaceutical and Biomedical Analysis, 51, 268-272, 2010
K. Hamada, T. Zhang, J. Desaki, K. Nakashiro, H. Itoh, K. Tani, Y. Koyama, H. Hamakawa, Carrier cell-mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinoma antigen 1 promoter-driven oncolytic adenovirus, The Journal of Gene Medicine, 12, 545–554, 2010
T. Ito, N. Iida-Tanaka, Y. Koyama, Efficient in vivo Gene Transfection by Stable DNA/PEI Complexes Coated by Hyaluronic Acid, Journal of Drug Targeting, 16, 276-281, 2008
M. Sakae, T. Ito, C. Yoshihara, N. Iida-Tanaka, H. Yanagie, M. Eriguchi, Y. Koyama, Highly efficient in vivo gene transfection by plasmid/PEI complexes coated by anionic PEG derivatives bearing carboxyl groups and RGD peptide, Biomedicine & Pharmacotherapy, 62, 448-453, 2008
M. Hashimoto, Y. Koyama, T. Sato, In vitro Gene Delivery by pDNA/Chitosan Complexes Coated with Anionic PEG Derivatives That Have a Sugar Side Chain, Chem Lett., 37, 266-267, 2008
免疫治療製剤
発明者：小山義之 出願日2015年11月30日 特願2015－244605
ハイドロゲル形成材
発明者：小山義之、伊藤智子 出願日2013年5月24日 特願2013－109515
ハイドロゲル形成材
発明者：小山義之、伊藤智子 出願日2012 年10 月23日 特願2012-233453
新規な複合体、それを含有する医薬及び癌の治療方法
発明者：小山 義之、芳原 智恵子 出願日2011年8月29日 特願2011－201136
新規なベクター、それを含有する医薬及び癌の治療方法
発明者：濱田 雄行、小山 義之、芳原智恵子、伊藤智子 出願日2010年4月20日 特願2010－20766
ウィルスの複合体、それを含有する治療薬及び治療方法
発明者：小山 義之、芳原 智恵子、濱田 雄行 出願日2010年4月28日
PCT/JP2010/058236 2010.4.28
遺伝子治療剤
発明者：濱田 雄行、小山 義之、芳原 智恵子 出願日2009年1月30日 特開2010-173990
核酸複合体徐放担体
発明者：小山 義之、芳原智恵子、奥富さやか 出願日2009年7月2日 公開2011-012041